Abstract
The ethyl acetate extract of Punica granatum fruits was fractionated by chromatographic techniques to afford the ellagitannin punicalagin. The substance was found to be active against methicillin-resistant Staphylococcus aureus strains and was identified by HPLC/UV and 1HNMR. The antibacterial assays which guided the isolation of the tannin were conducted using the disc diffusion method. Minimum inhibitory concentration (MIC) was determined by the dilution method according to NCCLS (National Committee for Clinical Laboratory Standards) procedure.
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Machado, T. D. B., Leal, I. C. R., Amaral, A. C. F., Dos Santos, K. R. N., Da Silva, M. G., & Kuster, R. M. (2002). Antimicrobial ellagitannin of Punica granatum fruits. Journal of the Brazilian Chemical Society, 13(5), 606–610. https://doi.org/10.1590/S0103-50532002000500010
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