Detection and isolation of β-conglycinin-susceptible gut indigenous bacteria from ICR mice fed high-sucrose diet

Abstract

β-Conglycinin (BC), a principal protein component of soybeans, exhibits antioxidant, antibacterial, and immunomodulatory properties. In this study, the presence of BC-susceptible gut indigenous bacteria (SIB) was examined in Institute of Cancer Research (ICR) mice fed either 20% (w/w) soy protein (SP), BC, or milk casein (MC) for 14 days. The body weight, liver weight, and plasma glucose levels were lower in the BC group than in the MC group. Caecal microbiome was analysed using 16S rRNA gene (V4) amplicon sequencing, the QIIME2 bioinformatics pipeline, and the SILVA database. While Muribaculum sp.- and Roseburia-like bacteria were abundant, Eisenbergiella-, Desulfovibrionaceae-, Mucispirillum schaedleri-, and Bacteroides massiliensis-like bacteria were less frequent in the SP and BC groups. Furthermore, Tidjanibacter massiliensis-, Duncaniella sp.-, and Enterorhabdus-like bacteria were higher in the BC group than in the MC and SP groups. Adlercreutzia caecimuris (formerly named Enterorhabdus caecimuris) could be isolated using blood liver agar plates. These bacteria regarded as BC-SIB might exert interactive effects upon consumption of soybeans or SP and thereby influence the host health.