An Interpretation of the Apparent Dual Specificity of Some Murine Myeloma Immunoglobulins with Inulinbinding Activity

Abstract

Five murine myeloma immunoglobulins have been studied for their binding with fructose-containing homopolysaccharides. The results indicate that the anti-(2→1)-D-fructofuranans are capable of binding (2→6)-D-fructofuranans with reduced affinity. An anti-(2→6)-D-fructofuranan (U10) was incapable of binding a (2→1)-D-fructofuranan. By using molecular models of fructofuranans of both linkage types, and a hypothetical molecular model of the combining region of E109, an anti-(2→1)-D-fructofuranan, a rationalization of these data can be proposed.