IL-4-Producing γδ T Cells That Express a Very Restricted TCR Repertoire Are Preferentially Localized in Liver and Spleen

Abstract

IL-4-producing γδ thymocytes in normal mice belong to a distinct subset of γδ T cells characterized by low expression of Thy-1. This γδ thymocyte subset shares a number of phenotypic and functional properties with the NK T cell population. Thy-1dull γδ thymocytes in DBA/2 mice express a restricted repertoire of TCRs that are composed of the Vγ1 gene product mainly associated with the Vδ6.4 chain and exhibit limited junctional sequence diversity. Using mice transgenic for a rearranged Vγ1Jγ4Cγ4 chain and a novel mAb (9D3) specific for the Vδ6.3 and Vδ6.4 murine TCRδ chains, we have analyzed the peripheral localization and functional properties of γδ T cells displaying a similarly restricted TCR repertoire. In transgenic mice, IL-4 production by peripheral γδ T cells was confined to the γδ+9D3+ subset, which contains cells with a TCR repertoire similar to that found in Thy-1dull γδ thymocytes. In normal DBA/2 mice such cells represent close to half of the γδ T cells present in the liver and around 20% of the splenic γδ T cells.