THE BIOSYNTHESIS OF SILVER NANOPARTICLES BY CYTOPLASMIC FLUID OF CORIOLUS VERSICOLOR

Abstract

The aim of this study is to investigate the production conditions of silver nanoparticles (NPs) in the presence of AgNO3 with the fungal cytoplasmic fluid (FCF) of white rot fungus Coriolus versicolor. In this study, parameters such as pH, AgNO3 concentration and FCF ratio of C. versicolor were optimized. C. versicolor was grown in SBM and then kept in ultrapure water to obtain FCF for synthesis of the nanoparticle. Nanoparticle formation was monitored by UV spectrophotometry at 420 nm wavelength and the silver nanoparticles were imaged by SEM. In the optimization study, it was found that at pH 5.0, 1.5 mM AgNO3 and 50% FCF containing medium was found to provide optimal conditions for the synthesis of the silver nanoparticle. The nanoparticles were spherical and varied between 15-35 nm.