Microplate-based assay for screening of advanced glycation end products binding to its receptor

Abstract

Advanced Glycation End products (AGEs) are a group of amino-acid modifications produced with sugars or di-carbonyls. Some AGEs are known to affect health through binding to the receptor of AGEs (RAGE). Here, we propose a method for screening RAGE-binding AGEs by a competitive assay using purified RAGE and AGEs-specific antibody. This method has clarified that at least carboxyethyl lysine and pentosidine among methylglyoxal-derived AGEs are involved in RAGE binding, suggesting that this would be a promising method for classifying RAGE-binding AGEs.