Development and validation of RP-HPLC method for the estimation of alfuzosin hydrochloride in bulk drug and tablets

Abstract

A reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the estimation of Alfuzosin hydrochloride in bulk drug and pharmaceutical dosage form. The quantification was carried out using a RP stainless steel column in isocratic mode, with mobile phase consisting of methanol, water, triethyl amine in the ratio of 60: 39.6: 0.4 (v/v) and added concentrated formic acid to make up the pH 4. The mobile phase was pumped at a rate of 0.9 mL/min and the detection was carried out at 245 nm and the linearity was found to be in the range of 10 to 1000 mcg/mL. The proposed method was found to be simple, precise, accurate, rapid and reproducible for the estimation of Alfuzosin hydrochloride in bulk drug and tablets.