New Efficient Recombinant Expression System to Engineer Candida antarctica Lipase B

Abstract

Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme. Copyright © 2010, American Society for Microbiology. All Rights Reserved.