Ciri Adaptasi Anatomi Daun Bougainvillea spectabilis W. Yang Dijangkiti Virus Bougainvillea chlorotic vein-banding

Abstract

Leaf anatomy studies were conducted on Bougainvillea spectabilis infected with Bougainvillea chlorotic vein-banding (BCVB) virus. The objective of the study is to identify the anatomical adaptation characteristics of B. spectabilis leaves infected with the BCVB virus through comparison with the anatomical characteristics of control leaves and to identify cells and tissues that are adapted to the presence of the virus. The research method involves sectioning with a sliding microtome on the lamina and margin, petiole and midrib, epidermal peels of the adaxial and abaxial epidermis, leaf clearing to observe the venation, staining, mounting, and observation under a light microscope. The results of the study show that in infected leaves, the chlorophyll content is very low in the lamina and leaf margin, an increase in the number of vascular bundles, vascular tissue, and collenchyma cells, as well as cell wall damage. The results of observation of the anatomical characteristics of the abaxial epidermis of leaves show that leaves infected with the virus have a lower index number, and size of stomata compared to healthy leaves. The results of the study also proved changes in the shape of the abaxial epidermal wall and the thickening of the adaxial epidermal cell wall of leaves infected with the BCVB virus. The presence of crystals and tertiary veins is less clearly observed in virus-infected leaves. Changes in cells and tissues in leaves infected with BCVB virus are a response to infection and leaf adaptation to virus infection. The results of this study can be used as a reference to detect BCVB virus infection and control methods that can be performed on infected cells and tissues.