Simple and Highly Selective Electrochemical Sensor Constructed Using Silver Molybdate Nano-Wire Modified Electrodes for the Determination of Oxidative Stress Biomarker in Blood Serum and Lens Cleaning Solution

Abstract

In this work, a simple synthesis of (silver molybdate) Ag 2 MoO 4 nanowires was developed by varying the pH. By adjusting the pH, agglomeration of each Ag 2 MoO 4 nanowires were seen and electrochemical performance over each pH was investigated. To construct Ag 2 MoO 4 nanowires, this process involves a co-precipitation assisted ultrasound reaction. The as-synthesized Ag 2 MoO 4 was physically characterized using SEM, elemental mapping, EDX, and XRD analysis. Ag 2 MoO 4 modified GCE was developed to determine a hydrothermal free and urea-free assisted synthesis. H 2 O 2 plays a major role in the physiological process and it’s an oxidative stress biomarker. Here we develop an electrochemical detection of enzyme-free H 2 O 2 by amperometric method. The results obtained by modifying the electrode Ag 2 MoO 4 @pH-1 (AM@1) showed a good electrochemical reduction over current when compared to the bare electrode and Ag 2 MoO 4 @pH 2 to 5. LOD was calculated to be 5.42 nM with a linear range from 0.015 to 799.2 μ M. The selectivity of sensor was analysed based on AM@1 modified electrode and also, the modified AM@1/GCE sensor has been practically applied in biological samples. Further, AM@1 showed a greater selectivity towards H 2 O 2 in presence of some other biochemicals. Moreover, AM@1 exhibits advantages such as good stability, reproducibility, and repeatability.