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Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae

Genetics (2006) 172(1) 709-711
DOI: 10.1534/genetics.105.047662
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Abstract

Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3Δ1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his51 gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA. Copyright © 2006 by the Genetics Society of America.

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APA

Daniel, J. A., Yoo, J., Bettinger, B. T., Amberg, D. C., & Burke, D. J. (2006). Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae. Genetics, 172(1), 709–711. https://doi.org/10.1534/genetics.105.047662

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