Antioxidant activity, phenol and flavonoid content, and formulation cream of Stevia rebaudiana Bert

Abstract

The presented study aimed to characterize the content, biological activity and formulation of extracts prepared from dried Stevia rebaudiana Bert leaves. Stevia rebaudiana Bert is the leaves that have antioxidant activity. The leaves, stems and flowers of stevia contain a complex mixture of sweet diterpene glycosides, including isosteviol, stevioside, rebaudioside (A, B, C, D, E, and F), steviolbioside and dulcoside A. In addition to glycosides, the leaves of stevia also contain other phytoconstituents, such as flavonoids, phenolic acids, fatty acids, proteins, and vitamins. These compounds can prevent and retain free radicals substitution, which can cause aging and chronic diseases. The extract obtained was filtered and evaporated-determination of total phenolics and total flavonoid using spectrophotometric methods. The antioxidant potential of this extract was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. In the DPPH radical-scavenging activities, the extract had the antioxidant activity (IC50 = 48.18 μg/ml). The results showed that these extracts of Stevia rebaudiana Bert could be considered as natural antioxidants. The evaluation of the formulated cream showed good results and can be good potential for cosmetic product development.