Isolation and characterization of polymorphic microsatellite loci in Camellia Nitidissima Chi (Theaceae)

Abstract

Premise of the study: Microsatellite markers were developed in Camellia nitidissima for further population genetic studies. Methods and Results: Eight microsatellite markers were newly developed from C. nitidissima and 7 were transferred from other Camellia species. Two to 13 alleles per locus were identified for these microsatellites. Observed and expected heterozy-gosities ranged from 0.040 to 0.909, and 0.184 to 0.916, respectively. Four loci showed a significant deviation from Hardy-Weinberg equilibrium and five locus pairs displayed linkage disequilibrium. Conclusions: These microsatellite markers will be useful to assess the genetic variation and genetic structure of C. nitidissima. © 2010 Botanical Society of America.