Fluorescence micro-optical sectioning tomography using acousto-optical deflector-based confocal scheme

  • Qi X
  • Yang T
  • Li L
  • et al.
9Citations
Citations of this article
28Readers
Mendeley users who have this article in their library.

Abstract

© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE). Fluorescent labeling has opened up the possibility of clarifying the complex distribution and circuit wiring of specific neural circuits for particular functions. To acquire the brain-wide fluorescently labeled neural wiring, we have previously developed the fluorescence micro-optical sectioning tomography imaging system. This employs simultaneous mechanical sectioning and confocal imaging of the slices, and is capable of acquiring the image dataset of a centimeter-sized whole-mouse brain at a voxel resolution of 1 μm. We analyze the key optical considerations for the use of an acousto-optical deflector (AOD) scanner-based confocal detection scheme in this system. As a result, the influence of confocal detection, the imaging site during sectioning, and AOD fast scan mode on signal-to-background noise ratio are described. It is shown that mechanical sectioning to separate the slice and optical sectioning by confocal detection should be combined to maximize background suppression in simultaneous fast scan imaging while sectioning system setup.

Cite

CITATION STYLE

APA

Qi, X., Yang, T., Li, L., Wang, J., Zeng, S., & Lv, X. (2015). Fluorescence micro-optical sectioning tomography using acousto-optical deflector-based confocal scheme. Neurophotonics, 2(4), 041406. https://doi.org/10.1117/1.nph.2.4.041406

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free