In Vitro Antioxidant Properties of Mitratonic Remedy in Tea Form and In Vivo Protection Against Methanol-Induced Oxidative Stress in Wistar Rats

Abstract

Natural healthcare products with antiaging properties are attracting growing interest, and Thailand has a rich tradition of using herbal remedies for disease treatment and overall well-being. Mitra tea, derived from the traditional Mitratonic Remedy, is a Thai herbal formulation that has been used by local traditional healers for over 20 years and is officially recognized by the Department of Thai Traditional and Alternative Medicine, Ministry of Public Health, Thailand. Traditionally, it has been used to promote vitality, strengthen the body, and support longevity. However, scientific evidence supporting these traditional claims remains limited. Therefore, this study aimed to investigate the potential of Mitratonic Remedy in the form of herbal tea, herein referred to as Mitra tea. This study evaluated the antioxidant activity of Mitra tea through various antioxidant in vitro assays and analysis, alongside an in vivo male Wistar rats model with induced oxidative stress. Results demonstrated remarkably strong antioxidant properties, with notable DPPH scavenging activity (0.40 mg/mL) and high ferric-reducing antioxidant power (FRAP: 2551.95 mM FeSO4 equivalent/mg sample). Additionally, Mitra tea exhibited exceptional phenolic (142.76 mg/g) and flavonoid content (11,432.34 mg/g), further reinforcing its bioactive potential. In vivo findings revealed its ability to reduce MDA levels while enhancing key antioxidant enzymes, suggesting its effectiveness in mitigating oxidative stress. With its significant antioxidant properties and potential health benefits, Mitra tea stands out as a promising natural healthcare solution for antiaging product development, offering a scientifically backed alternative for consumers seeking longevity and wellness.