Effect of the Exposure of Aloe Vera Polyphenol Fraction on Changes in Blood Glucose of Koi Fish (Cyprinus carpio) as a Stress Response

Abstract

Aloe vera polyphenols can cause stress in fish, which results in changes in the blood such as increased blood glucose in fish. The purpose of this study was to analyze the blood glucose parameters of koi fish after being exposed to the polyphenol fraction of A. vera. The method used, previously observed that LC 50% resulted in 150 mg/kg treatment causing 50% death so the dose of A. vera fraction injected into koi fish muscles by the treatment: Control (-) without treatment, Control (+) given tannin/synthetic polyphenols compound 3 mg/kg Biomass Weight of koi fish, treatment A=75 mg/kg BW, B= 100mg/kg BW, C= 125 mg/kg BW. After being injected, starting 72 hours the koi fish blood plasma was taken to see the effect of stress on koi fish by looking at blood glucose parameters. The results of blood glucose parameters were getting higher (from 83,33 mg/dl to 132,33 mg/dl) when exposed to polyphenol fractions. Clinical symptoms after being injected with the polyphenolic fraction of A. vera caused the fish to swim abnormally, with red spots, pale, and hemorrhages.