Observation of Growth of Human Fibroblasts on Silver Nanoparticles

Abstract

Silver nanoparticles have drawn extensive attention as biomaterial components. Human fibroblasts were grown on various concentrations of silver nanoparticles during the period observation. Normal viability (0% silver particles) was increased from 6 to 72 hours, increasing the amount of human fibroblasts (1.5 × 104 to 7 × 106 cells/well) normally. Nevertheless, at higher concentrations of silver nanoparticles (50%) 1.11 × 105 cells/well remained after 72 hours. Results indicated that the increase in the concentration of silver nanoparticles reduced the number of fibroblasts and affected their fission. Silver nanoparticles were found under the membranes of fibroblast following dry treatment. The number of tissues declined because the silver nanoparticles interrupted the fission mechanism during their development in vivo. © 2007 IOP Publishing Ltd.