Characterization of Biogenic Silver Nanoparticles by Salvadora persica Leaves Extract and its Application Against Some MDR Pathogens E. coli and S. aureus

Abstract

Background: Now a days, the multidisciplinary scientific research conception in the field of nanotechnol. has witnessed development with regard to the numerous applications and synthesis of nanomaterials. Objective: The current investigation has been conducted with the main focus on the green synthesis of silver nanoparticles from the leaves of Salvadora persica and its antibacterial activity against MDR pathogens E. coli and S. aureus. Methodol.: Silver nanoparticles (AgNPs) were prepd. after addn. of aq. ext. of Salvadora persica leaves. The UV-Vis spectrophotometer, Transmission Electron Microscopy (TEM), zeta potential and SEM (SEM) were employed to detect the particle size and morphol., besides Fourier transform infra-red spectrometer (FTIR) anal. was performed to det. the capping and stabilizing agents in the ext. Antibacterial assay for the biogenic AgNPs was conducted against E. coli and S. aureus. Results: Color change of the mixt. from yellow to dark brown is the first indication to AgNPs formation. Furthermore, 420 nm was the peak value for UV-Vis spectroscopy absorption of the mixt. Besides, TEM and SEM micrographs showed wide variability in the diam. of smaller NPs aggregated together with spherical shapes and zeta sizer showed about 153.3 nm as an av. size of nanoparticles. Microbial suppression was noticed for the tested microorganisms. Furthermore, with the help of FTIR anal. the biomols. that act as capping and stabilizing agents of AgNPs are proteins and phenols present in the plant ext. Conclusion:Salvadora persica leaves ext. act as a reducing and stabilizing agent for the synthesis of AgNPs keeping its ability to suppress the MDR pathogen. [on SciFinder(R)]