UJI TOKSISITAS DAN SKRINING FITOKIMIA EKSTRAK TUMBUHAN SUMBER PAKAN ORANGUTAN TERHADAP LARVA

Abstract

Toxicity test and phytochemical screening for orangutan food extracts were done on some species including Dacryodes rugosa (Blume) H.J. Lam., Durio acutifolius (Mast.) Kosterm., Madhuca sericea H.J. Lam., Triomma malaccensis Hook. F., Sandoricum koetjape (Burm. f.) Merrill, and Scaphium macropodum (Miquel) Beumee ex Heyne. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Samples of leaves of orangutan foods taken from the Gunung Beratus Protected Forest have been repeatedly extracted with methanol. The samples were then concentrated using a rotary evaporator until a crude extract was obtained. Each extract was tested by toxicity to larva Artemia salina L. The toxicity test for larvae A. salina have been done at seven concentration levels, i.e: 1,000 ppm, 500 ppm, 250 ppm, 125 ppm, 62.5 ppm, 31.2 ppm, and 15.6 ppm. The result of the research showed that extract of D. rugosa had the highest toxicity among all the other extracts. The lowest LC 50 value, i.e. 125.57 ppm, was shown by A. salina at the concentration of 1,000 ppm and mortality rate of 87%.