Performance of Loop-Mediated Isothermal Amplification (LAMP) Targeting the Nucleocapsid (N) Gene of SARS-CoV-2 for Rapid Diagnosis of COVID-19: Systematic Review and Meta-Analysis

Abstract

SARS-CoV-2 emerged as a new respiratory virus spreading rapidly to all areas of the world. A systematic review with meta-analysis concerning the use of Loop-Mediated Isothermal Amplification (LAMP) methodology targeting the SARS-CoV-2 nucleoprotein (N) gene was conducted. The search resulted in 229 articles, of which 19 articles were selected to compose the final review. In general, LAMP showed a high specificity in the detection of SARS-CoV-2 and a wide variation of sensitivity values. The LAMP method was considered a fast and highly specific method for SARS-CoV-2 detection; however, some variables may affect its sensitivity and overall performance.