A validated HPLC method for analysis of Atorvastatin Calcium, Ramipril and Aspirin as the bulk drug and in combined capsule dosage forms

Abstract

A simple, fast, accurate and precise method has been developed for the simultaneous determination of Atorvastatin Calcium (ATR), Ramipril (RAM) and Aspirin (ASP) from pharmaceutical formulation by high performance liquid chromatography (HPLC). The separation was carried out on C-18 column using Methanol and Acetate buffer [pH adjusted to 3.1 with Orthophosphoric acid (dil.)] in the ratio (70:30v/v). The retention times of Atorvastatin Calcium (ATR) - 8.38 ± 0.10 min, Ramipril (RAM) - 5.62 ± 0.02 min, (ASP) - 3.04 ± 0.15 min. The developed method was validated as per ICH Guidelines.