Bacterial growth and substrate degradation by BTX-oxidizing culture in response to salt stress

Abstract

Interactions between microbial growth and substrate degradation are important in determining the performance of trickle-bed bioreactors (TBB), especially when salt is added to reduce biomass formation in order to alleviate media clogging. This study was aimed at quantifying salinity effects on bacterial growth and substrate degradation, and at acquiring kinetic information in order to improve the design and operation of TBB. Experiment works began by cultivating a mixed culture in a chemostat reactor receiving artificial influent containing a mixture of benzene, toluene, and xylene (BTX), followed by using the enrichment culture to degrade the individual BTX substrates under a particular salinity, which ranged 0-50 g l-1 in batch mode. Then, the measured concentrations of biomass and residual substrate versus time were analyzed with the microbial kinetics; moreover, the obtained microbial kinetic constants under various salinities were modeled using noncompetitive inhibition kinetics. For the three substrates the observed bacterial yields appeared to be decreased from 0.51-0.74 to 0.20-0.22 mg mg-1 and the maximum specific rate of substrate utilization, q̂, declined from 0.25-0.42 to 0.07-0.11 h-1, as the salinity increased from 0 to 50 NaCl g l -1. The NaCl acted as noncompetitive inhibitor, where the modeling inhibitions of the coefficients, KT(S), were 22.7-29.7 g l -1 for substrate degradation and KT(μ), 13.0-19.0 g l-1, for biomass formation. The calculated ratios for the bacterial maintenance rate, mS, to q̂ further indicated that the percentage energy spent on maintenance increased from 19-24 to 86-91% as salinity level increased from 0 to 50 g l-1. These results revealed that the bacterial growth was more inhibited than substrate degradation by the BTX oxidizers under the tested salinity levels. The findings from this study demonstrate the potential of applying NaCl salt to control excessive biomass formation in biotrickling filters. © Society for Industrial Microbiology 2005.