In-situ Glycine Sensor Development Based ZnO/Al2O3/Cr2O3 Nanoparticles

Abstract

Initially, low-dimensional ternary nanoparticles of ZnO/Al2O3/Cr2O3 were synthesized using reliable solvothermal process and successfully implemented to fabricate enzyme-less selective glycine (Gly) sensor. The calibration curve was plotted from the linear relation of current versus concentration of glycine. By using the slope of resultant calibration curve, the sensitivity (2.09*102 μAμM−1cm−2) of Gly sensor was estimated by considering the active surface area of fabricated sensor probe. The proposed enzyme-free Gly biosensor was found linear over large concentration range of 0.1 nM ∼ 1.0 μM, known as linear dynamic range (LDR). The detection limit (82.25±4.11 pM) was calculated at signal to noise ratio of 3 from the calibration curve. The proposed enzyme-less Gly sensor was exhibited good reproducibility, long-term stability and efficiency to detect Gly in real biological samples by electrochemical approach. Thus, this novel research methodology might be a good technique for the development of enzyme-less sensor using ternary metal oxide nanoparticles onto GCE for the biomedical applications.