Complex of intratumoral mycobiome and bacteriome predicts the recurrence of laryngeal squamous cell carcinoma

Abstract

Dysbiosis of intratumoral fungal and bacterial communities is associated with poor prognosis in various cancers. However, the mycobiome characteristics in laryngeal squamous cell carcinoma (LSCC) and its correlation with recurrence have not yet been investigated. The mycobiome in 80 LSCC samples was characterized using internal transcribed spacer sequencing, encompassing both tumor tissues and their matched para-cancerous tissues. The intratumoral bacteriome was further identified using 16S rRNA sequencing. These two microbial communities were analyzed using bioinformatics and statistical methods to determine its potential correlation with LSCC recurrence. The fungal alpha diversity in tumors was higher compared with that in para-cancerous tissues (P < 0.001). A significant difference in the overall fungal community patterns between tumor tissues and para-cancerous tissues was observed based on Bray-Curtis dissimilarity (P < 0.001). The presence of Alloprevotella, Porphyromonas, Candida, and Fusarium in tumors exhibited a correlation with alcohol consumption. The relative abundance of Penicillium, Exophiala, and Aspergillus in the mycobiome, as well as that of Alloprevotella, Porphyromonas, and Peptostreptococcus in the bacteriome significantly increased the risk of LSCC recurrence (P < 0.05). These six microorganisms can combine to form a microbial complex, which may independently contribute to recurrence risk in patients with LSCC when enriched within the tumor (hazard ratio = 6.844, P < 0.01). Intratumoral fungi and bacteria can be valuable indicators for assessing recurrence in patients with LSCC, indicating their potential as valuable targets for therapeutic intervention.