TRIM33 gene somatic mutations identified by next generation sequencing in neoplasms of patients with anti-TIF1γpositive cancer-associated dermatomyositis

Abstract

Objective: To deep sequence the TRIM33 gene in tumours from patients with cancer-associated anti-TIF1γautoantibody-positive dermatomyositis (DM) as TRIM33 somatic mutations in tumours may trigger this auto-immune disease. Methods: Next generation sequencing of tumour DNA samples from patients with cancer-associated anti-TIF1γautoantibody-positive DM. Fourteen tumours from 13 anti-TIF1γautoantibody-positive DM individuals were sequenced along with two control tumours from non-DM individuals. Results: Fourteen probable somatic variants from four tumours were identified in the TRIM33 gene. Conclusion: These results are in accordance with the previous report of Pinal-Fernandez et al. and support the hypothesis of a role of TRIM33 gene mutations in the pathophysiology of anti-TIF1γautoantibody-positive DM.