Dual antennular chemosensory pathways can mediate orientation by Caribbean spiny lobsters in naturalistic flow conditions

Abstract

Benthic crustaceans rely on chemical stimuli to mediate a diversity of behaviors ranging from food localization and predator avoidance to den selection, conspecific interactions and grooming. To accomplish these tasks, Caribbean spiny lobsters (Panulirus argus) rely on a complex chemosensory system that is organized into two parallel chemosensory pathways originating in diverse populations of antennular sensilla and projecting to distinct neuropils within the brain. Chemosensory neurons associated with aesthetasc sensilla project to the glomerular olfactory lobes (the aesthetasc pathway), whereas those associated with non-aesthetasc sensilla project to the stratified lateral antennular neuropils and the unstructured median antennular neuropil (the non-aesthetasc pathway). Although the pathways differ anatomically, unique roles for each in odor-mediated behaviors have not been established. This study investigates the importance of each pathway for orientation by determining whether aesthetasc or non-aesthetasc sensilla are necessary and sufficient for a lobster to locate the source of a 2 in-distant food odor stimulus in a 5000-liter seawater flume under controlled flow conditions. To assess the importance of each pathway for this task, we selectively ablated specific populations of sensilla on the antennular flagella and compared the searching behavior of ablated animals to that of intact controls. Our results show that either the aesthetasc or the non-aesthetasc pathway alone is sufficient to mediate the behavior and that neither pathway alone is necessary. Under the current experimental conditions, there appears to be a high degree of functional overlap between the pathways for food localization behavior.