R & D of biofet for biological monitoring(I) development of new nitrate ISFETs.

Abstract

To develop high performance nitrate ion-selective field-effect transistors (ISFETs) to detect a human salivary nitrate, we investigated highly sensitive, selective and stable NO3--ISFETs using newly designed copper(I) complexes with 4 kinds of phenanthroline derivs. and 1 kind of available phenanthroline deriv., such as 2,9-dibutyl-1,10-phenanthroline (dbp), 2,9-diphenyl-1,10-phenanthroline (dpp), 2,9-dibutyl-4,7-diphenyl-1,10-phenanthroline (dbdpp), 2,4,7,9-tetraphenyl-1,10-phenanthroline (tpp) and also 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline (bcp) as a nitrate-sensing material. The NO3- ion-selective electrodes (ISEs) based on [Cu(bcp)2]NO3, [Cu(dbdpp)2]NO3 and [Cu(tpp)2]NO3 showed excellent sensitivity and reproducibility over conventional NO3--ISEs. We prepd. prototype of FET NO3- checker using available FET pH checker. We preliminarily applied human saliva after pretreatment using a cartridge column packed with cation-exchange resin of the silver form using direct potentiometry. We obtained relatively good correlation with conventional ion chromatog. [on SciFinder(R)]