A Rapid and Sensitive Early Diagnosis of Influenza Virus Subtype via Surface Enhanced Raman Scattering

Abstract

We have developed a novel, rapid, highly sensitive biosensor protocol for diagnosis and characterization of influenza viruses in the early stage of the epidemic. Using Surface-Enhanced Raman Scattering (SERS) technique and nitrocellulose membrane, detection limit as low as 30 ng/mL was achieved with high selectivity to different subtypes of influenza virus tested. Moreover, the SERS measurement can be completed in less than 2 h, thus making the protocol useful in early diagnosis and impediment of propagation of potential influenza pandemic.