ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF PLANT-MEDIATED GREEN SYNTHESIZED SILVER NANOPARTICLES USING Cissus quadrangularis AQUEOUS EXTRACT

Abstract

Plant-based green-synthesized silver nanoparticles (AgNPs) are valuable because of their advantages over traditional methods. This study examines the environment-friendly synthesized AgNPs using Cissus quadrangularis (CQ) stem aqueous extract. The optimum parameters for AgNP synthesis were a volume of plant extract (15 mL), aq. AgNO3 concentration (0.10 mM), AgNO3 volume (20 mL), reaction time (10 min), pH (11), and temperature (45oC). The synthesized AgNPs at optimum conditions were characterized through UV-Visible spectroscopy, FTIR, XRD, and SEM. In colloidal solutions, the occurrence of CQ-AgNP was studied through the color change measured by the UV-visible spectrum, which confirms the developed AgNPs with a typical resonance spectrum (at 430 nm). The percentage yield of synthesized AgNPs was also estimated as 94.32%. The FTIR spectra exhibit the availability of CQ phytochemicals responsible for the reduction, capping, and stabilization of Ag+ of CQ-AgNP. The XRD pattern and SEM results show that the generation of highly crystalline spherical shapes of AgNPs has a mean diameter of 8.68 nm. Also, CQ-AgNPs had significant antibacterial activity against S. aureus and E. coli. The CQ-AgNPs had better antioxidant activity compared to the ascorbic acid standard. Thus, this study recommends that the presently studied CQ-AgNPs may be used as a remedial agent for biomedical applications.