The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers

Abstract

Carbon source is an indispensable factor for the development of an in vitro morphogenic program of chrysanthemum micropropagation. The choice of carbon source affects the qualitative and quantitative outcome, and also the response of thin cell layers when these are placed onto morphogenic (callus, root, shoot, somatic embryo) media. Threshold survival levels (TSLs) could not be obtained for sucrose, fructose or glucose. TSLs together with organ differentiation were, however, obtained for mannose (60-80 g.L-1), xylose and lactose (40 g.L-1) and cellulose (60-80 g.L-1), making these carbon sources suitable as potential carbon sources for positive selection systems for chrysanthemum genetic transformation.