Biotransformation of benzaldehyde to L-phenylacetylcarbinol (L-PAC) by free cells of Torulaspora delbrueckii in presence of beta-cyclodextrin

Abstract

Studies were carried out to explore the possibility of decreasing the toxic and inhibitory effects of the substrate benzaldehyde during its biotransformation to L-PAC by free cells of Torulaspora delbrueckii using β-cyclodextrin (β-CD). Use of various levels of benzaldehyde and acetaldehyde in presence of 2% of β-CD showed that, in presence of β-CD, the organism could tolerate higher levels of benzaldehyde and acetaldehyde. Semi-continuous feeding of benzaldehyde and acetaldehyde was found to increase the yield of L-PAC in comparison with one time feeding.