Karst landforms as microrefugia for soil Collembola: Open versus forested dolines

Abstract

Enclosed surficial karst dolines are geomorphologically diverse habitats with the potential to strongly shape community structure and the distribution of functional groups of organisms. Forest habitats in karst landscapes are characterized by lower soil temperatures and microclimatic fluctuations compared to open habitats; therefore, they may provide favorable conditions for cold-adapted/psychrophilic species, which are highly vulnerable to climate warming. We compared the diversity and structure of soil Collembola communities at sites along microclimatic gradients in open and forested karst dolines. The effect of topography and related environmental conditions on the distribution of ecological groups of soil Collembola was analyzed, and the function of the dolines as potential climate refugia for psychrophilic arthropods was assessed. The karst dolines revealed significant habitat heterogeneity, including warm plateaus and S-facing slopes and colder and wetter bottoms and N-facing slopes. The inner sites of the dolines had an overall higher abundance and species richness compared to the plateau sites. Nonmetric multidimensional scaling ordination revealed a clear delimitation of Collembola communities in terms of warmer and cooler sites in the open dolines, while the forested dolines did not reveal such a clear pattern. The studied dolines showed a potential to serve as microrefugia in the context of global climate change. Dolines could support the long-term persistence of at least some species such as cold-adapted species. Karst dolines in the temperate zone play a key role in maintaining biodiversity; therefore, they should be central in biodiversity conservation programs.