Kinetic Parameters Determination Of Glucoamylase On Hydrolysis Reaction Of Sagoo Starch (Metroxylon sp)

Abstract

Sagoo plant (Metroxylon sp.) numerous in eastern Indonesia, such as Maluku and Papua. Sagoo starch containing amylose and amylopectin. Sagoo starch hydrolysis can be carried out by enzymes, such as glucoamylase or often called amiloglukosidase or α-1 ,4-glukanoglukohidrolase, an extracellular enzyme capable hydrolyzes α-1, 4 on amylose and α-1, 6 in amylopectin. The enzymatic hydrolysis of starch can be reviewed in the kinetics of the Michaelis-Menten equation and Lineweaver-Burk equation. The aims of this study to determine the kinetic parameters (Vmax and Km) of glucoamylase on hydrolysis of sagoo starch. Glucoamylase has an optimum temperature of 50 oC and pH optimum 6.5. At optimum conditions, based on Michaelis-Menten equation, the value of Vmax is 1.09 U/mL/min and Km value is 0.82. Based on Lineweaver-Burk equation, the value of Vmax was 1.03 U/ mL/min and Km value was 0.84%.