Development of a gold nanoparticle-based strip assay for detection of clopidol in the chicken

Abstract

In this study, a monoclonal antibody with selectivity for clopidol was produced based on a new hapten. The 50% inhibition concentration (IC50) for the developed ic-ELISA was 0.275 ng/mL. Cross-reactivity tests showed that the monoclonal antibody had high specificity for clopidol. Using this antibody, a lateral-flow colloidal gold immunoassay (LCGA) was created for the rapid screening of clopidol in raw chicken samples. With the naked eye, the cut-off value of the strip assay was 5 μg/kg for clopidol in chicken samples. Therefore, this LCGA provides a sensitive and rapid method for on-site detection of clopidol in chicken samples.