Chemical constituents from the roots of Polygala wattersii hance

Abstract

To investigate the chemical constituents of the roots of Polygala wattersii Hance, the separation and purification were performed by solvent extraction and repeated column chromatography (CC) on silica gel, Sephadex LH20 and macroporous resin D101, preparative TLC and semipreparative HPLC. The structures were identified by spectroscopic analysis and comparison of their 1 H and 13C NMR data with those reported in literatures. Twentythree known compounds, including eleven xanthones (1-11), nine sugar esters (12-20), two triterpenoid saponins (21 and 22) and one phenylpropanoid (23) were isolated and their structures were identified as 1,3-dihydroxyxanthone (1), 1-hydroxy3-methoxyxanthone (2), 1,3-dihydroxy-2-methoxyxanthone (3), 1,3,7-trihydroxy-2-methoxyxanthone (4), 1,3,6-trihydroxy-2,7-dimethoxyxanthone (5), 1,6,7-trihydroxy-2,3-dimethoxyxanthone (6), 1,7-dihydroxy-2,3-methylenedioxyxanthone (7), 1,7dimethoxyxanthone (8), 1,2,3-trimethoxyxanthone (9), 1methoxy2,3methylenedioxyxanthone (10), 6-hydroxy1methoxy-2,3-methylenedioxyxanthone (11), 3'Oferuloyl-6-O-acetyl sucrose (12), arillatose B (13), sibricose A5 (14), sibricose A6 (15), 3',6-diOsinapoyl sucrose (16), tenufoliside A (17), 3'O3,4,5-trimethoxycinnamoyl-6-O-pmethoxybenzoyl sucrose (18), glomeratose A (19), 1-O-p-coumaroylDglucopyranose (20), bayogenin-3-O-glucoside (21), tenufolin (22), and sinapic acid (23). Among them, compounds 2 and 12 were obtained from genus Polygala for the first time, and except compound 16, all others were isolated from this species for the first time.