Characterization and antagonistic effect of isolated Trichoderma sp. against pathogens under clusterbean (Cyamopsis tetragonoloba L.)

Abstract

A green house experiment was conducted at Department of Plant Pathology, College of Agriculture, Bikaner, to characterize the eight isolates of Trichoderma sp. (TH-8, TH-SOG, TH-HMG, TH-BKN, TH-SIK, TH-DUP, TH-JOB and TH-JAI) were collected from different locations of clusterbean (Cyamopsis tetragonoloba L.) fields in western Rajasthan and tested for their antagonistic property against soil borne plant pathogens. It was further studied for morphological and physiological characteristics such as color of mycelium, pigmentation of substrate, type, amount of mycelium growth, effect of temperature and pH. It was observed that, Potato Dextrose Agar (PDA) media, temp range 25-300C and pH 7.0-7.5 were found most effective for optimum growth and sporulation of TH-8. The isolate Trichoderma harzianum-8 (isolated from SKRAU Bikaner, Rajasthan) was found best up to 70 per cent of inhibition of mycelial growth of different clusterbean pathogen viz., Macrophomina phaseolina, Fusarium oxysporum, Sclerotinia sclerotiorum.