Monocyte tumor necrosis factor production in head and neck squamous cell carcinoma

Abstract

Changes in monocyte and macrophage function have been demonstrated in patients with head and neck squamous cell carcinoma. The purpose of this study was to evaluate tumor necrosis factor (TNT) production by activated monocytes from patients with head and neck squamous cell carcinoma and to evaluate its relation to cancer stage, weight loss, and performance status. Monocytes from patients (n = 10) and controls (n = 10) were isolated from peripheral blood mononuclear cells by plastic adherence and incubated with lipopolysaccharide (10 µg/mL). The TNF concentration of supernatants was assayed by TNFα-specific immunoassay. The TNF production by monocytes from head and neck squamous cell carcinoma patients was significantly higher (P