MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung's Disease

Abstract

Background: Hirschsprung's disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR.Methods: In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR and 66 controls without HSCR. Transwell, Cell Counting Kit-8 (CCK-8) and flow cytometry assay were employed to detect the function role of miR-195 in vitro.Results: Our results showed that expression levels of miR-195 from patients with HSCR were significantly higher than control group; along with aberrant lower expression levels of digestive-organ expansion factor (DIEXF) were tested. Increased level of miR-195 could suppress the level of DIEXF in cell, which induced the impairment of cell migration and proliferation.Conclusions: Aberrant expression of miR-195 may involved in the pathogenesis of HSCR by down-regulated the level of DIEXF. © 2014 Lei et al.; licensee BioMed Central Ltd.