In-vitro Interpretation of 8-OHdG Formation as Cancer Risk Biomarker due to Malondialdehyde (MDA) and Cr (VI) Exposure through Fenton-Like Reaction

Abstract

Malondialdehyde (MDA) has been widely reported as a biomarker. Endogenous genotoxic products formed from the results of lipid peroxidation and oxidative stress can bind and modify proteins, phospholipids, and DNA to form stable adducts. The increase of oxidative stress triggers how the adducts are formed-formation of adducts linked to various patterns of diseases such as cancer, cardiovascular, and neurodegenerative diseases. The research objective was to interpret 8-OHdG formation, which is triggered by oxidative damage of genetic code (2'-deoxyguanosine) exposure to chemical compounds MDA and chromium (VI). This research was done by using in-vitro studies using MDA compound and Fenton-Like reactions. The variation of reaction conditions were at 7.4 and 8.4 pH condition, 3 and 16 hours of the incubated period at the temperature of 37°C and 60°C. The reaction products were analyzed by HPLC. From this analysis, 8OHdG target compounds were found. The correlation coefficient's value is 0.9973. The value of LOD is 11.03 ?g/L, and the LOQ value is 36.77 ?g/L. In vitro test to MDA compounds at 60°C incubation temperature conditions for 3 hours, and pH 7.4 produced the highest concentration of 8-OHdG, 404,09 ?g/L.