Media for selective isolation of an entomogenous fungus, Beauveria bassiana (Deuteromycotina: Hyphomycetes)

Abstract

Growth of Beauveria bassiana at various pHs was investigated and the fungus was found to be able to grow well at high pHs of more than 10. The fungus was also resistant to CuCl2 and to low sugar content. New selective media for the isolation of B. bassiana were developed by integrating CuCl2, low sugar, and either crystal violet or brilliant green in the media. Beauveria bassiana was isolated from soil samples using the developed media, and isolation efficiency was found to be higher using one of our newly developed media than by using that of VEEN and FERRON which has commonly been used for the selective isolation of B. bassiana.