A confounding effect of bacterial titer in a type III delivery-based assay of eukaryotic effector function

Abstract

This letter describes a newly discovered confounding effect ofbacterial titer in a previously published type III delivery-basedassay of the fungal effector BEC1019. The original publication(Whigham et al. 2015) has been retracted as a consequence of thisdiscovery. Here, we tabulate the affected and unaffected figuresand conclusions in the original publication and briefly reflect onpotential pitfalls to bear in mind when designing experiments thatuse bacterial type III secretion to characterize eukaryotic effectors.