Detection of Glycosylated and Deglycosylated Extensin Precursors by Indirect Competitive ELISA

  • Conrad T
  • Lamport D
  • Hammerschmidt R
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Abstract

A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.

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Conrad, T. A., Lamport, D. T. A., & Hammerschmidt, R. (1987). Detection of Glycosylated and Deglycosylated Extensin Precursors by Indirect Competitive ELISA. Plant Physiology, 83(1), 1–3. https://doi.org/10.1104/pp.83.1.1

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