MITIGATION OF HEAVY METALS FROM THE SOIL THROUGH IN-VITRO AND IN-VIVO PRODUCED PLANTS OF IMPATIENTS BALSAMINA L.

Abstract

In this research Heavy Metal extraction capacity or phytoremediation capacity of Impatiens balsamina L. was assessed. Here, two different approaches In-vitro and In-vivo were used for the production of plantlets. In-vitro approach involved tissue culture approach and In-vivo direct through media (soil, cocopeat, mosses). Seeds were used for the production of plantlets. After 30 days of seedlings development all the plantlets which are produced through In-vitro and In-vivo approaches and plants were transplanted in the pots and treated with two metals Lead and Cadmium in the form of Pb (NO3)2 and Cd (NO3)2. Different concentrations were selected for Lead 200mg, 400mg, 600mg, 800mg/Kg and for Cadmium 5mg, 10mg, 15mg, and 20mg/Kg. Each pot was filled with 5Kg of soil. The metals were given directly through root zone of plants in solution form. After incubation time of 75 days mature and treated plants were collected and each and every part of every concentration treated plants were collected and AAS (Atomic Absorption Spectroscopy) was assessed. As compare to In-vivo produced plants In-vitro produced plants has more capacity to accumulate Lead and Cadmium.