Antipsoriatic and inhibitory effects of an oral dosage form containing bioflavonoids on inflammatory cytokines IL-1α, IL-1β, IL-6, IL-8, IL-17 and TNF-α

Abstract

Objective: The present study aimed to develop and evaluate orally administrable dosage form containing bioactive flavonoids viz., Luteolin-7-O-β-D-Glucuronide (II), Kaempferol 3-O-[2-O-(6-O-feruloyl)-β-D-glucopyranosyl]-β- D-galactopyranoside (III) from the bark of Givotia rottleriformis and Formononetin-7-O-β-D-glucoside (VI) from the leaves of Cassia tora. Methods: The formulation was developed and evaluated as per official compendia. The developed formulation was evaluated for antipsoriatic activity using rat UV ray photo dermatitis model and cytokine inhibition assay. Results: Evaluation parameters observed optimum range of formulations and the tablets were suitable for oral administration. The formulation exhibited antipsoriatic activity by good reduction in the thickness of epidermis, significant retention of the stratum granulosum and the absence of movement of neutrophils in UV-B induced psoriasis. In cytokine inhibition assay, the formulation showed remarkable inhibition of IL-17 and TNF- α, key cytokines involved in the pathogenesis of psoriasis at higher concentration. Conclusion: The study can be concluded that the developed formulation can be effective and safety as dietary supplements with health benefits to psoriatic patients.