Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Daun Anggur (Vitis vinifera L)

Abstract

ABSTRAK Telah dilakukan penelitian mengenai analisis kadar fenolik dan flavonoid total ekstrak daun anggur (Vitis vinifera L) dengan metode Spektrofotometri UV-Vis. Penelitian ini bertujuan untuk menentukan kadar fenolik dan flavonoid total dalam ekstrak etanol daun anggur. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol. Penentuan kadar fenolik total ekstrak menggunakan pembanding asam galat dengan beberapa variasi konsentrasi. Penentuan kadar flavonoid total menggunakan pembanding kuersetin. Nilai absorbansi diukur dengan spektrofotometer. Nilai absorbansi kemudian dimasukkan ke dalam persamaan regresi linier. Hasil penelitian menunjukkan kadar total fenolik dan kadar total flavonoid dalam ekstrak berturut-turut adalah sebesar 95,28 mg GAE/g dan 4,07 mg QE/g. Kata Kunci : Vitis vinifera, maserasi, kadar fenolik total, kadar flavonoid total ABSTRACT Studies on the analysis of the total phenol and flavonoid content of grapevine leaf extract (Vitis vinifera L) using UV-Vis spectrophotometry has been researched. The aim of this study is to determine the total content of phenol and flavonoids in the ethanol extract from leaves of Vitis vinifera L. The samples were extracted using the maceration method with ethanol as the solvent. Determination of total phenol content of extract using a gallic acid as standard with various concentration variations. Determination of total flavonoid content using quercetin as standard. Absorbances measured with spectrophotometer. Absorbances values then inserted a linear regression equation. The results showed that the total phenol and flavonoid contents of extract were, respectively, 95,28 mg GAE/g and 4.07 mg QE/g.