In Vitro antibacterial activity of Mindi (Melia azedarach Linn.) leaf extract with nanoencapsulation technology

Abstract

Purpose of this study was to examine the effect of nanoencapsulation technology in protecting the bioactive components of mindi (Melia azedarach Linn.) leaf extract. The first step was formulation of the ratio of mindi leaf extract:chitosan:sodium tripolyphosphate (STPP) for nanoencapsulation by ionic cross-linking. The results showed that the optimum formulation ratio of mindi leaf extracts:chitosan:STPP was 1:7:1/175. Nano-capsulation characteristics of the fitobiotic was measured (particle size (PSA), zeta potential value, morphology of nanoencapsulation (TEM), and bacterial growth inhibition). The second step was measuring the bacterial growth inhibition using the well diffusion technique with six treatments and three replications. The treatments were: Aquadest (T1), aquadest with 100 ppm antibiotic Tetracycline addition (T2), aquadest with 0,2% chitosan (T3), aquadest with 0,04% STPP (T4), aquadest with 2% mindi leaf extract (T5); and aquadest with nanoencapsulated mindi leaf extract (T6). The results showed that particle size of nanoencapsulation mindi leaf extract was 535.2±12.83 nm with Polydispersity Index (PI) 0.436±0,21 and zeta potential value 59.73±0.35 mV. The formulation of mindi leaf extract was found to possess inhibitory activity against Escherichia coli, Lactobacillus acidophilus, and Salmonella typhimurium. The research concluded that formulation of mindi leaf extract was clear, stabile and had antibacterial activity.