Microbes-Assisted Arsenate Reduction Activity in Bangladesh Drinking Water Wells as Revealed by Enrichment Cultivation

Abstract

Arsenate-reducing microorganisms may mobilize arsenic into groundwater. Phylogenetic DNA sequence information cannot exclusively be used to infer arsenate reduction, as the spotted homologies may not translate to biochemical activities. We here develop a cultivation-based metagenomic strategy for inferring actual activities: we complement a cultivation-independent microbial community survey covering 22 arsenic-contaminated drinking water wells in Bangladesh, with the characterization of enrichments of anaerobic arsenate reducers. All investigated samples revealed microbial arsenate reduction activity. The enriched microbial communities were phylogenetically diverse within and between the enrichments. The enrichment also unveiled species: the arsenate-reducing enrichments held 16S rRNA gene sequences closest to Dechloromonas sp., Azonexus fungiphilus, Youngiibacter fragile, Methanogenic prokaryote and Fusibacter sp., in addition to arsenate-reducing Sulfurospirillum strains NP4, b10 and dissimilatory iron-reducing Geobacter species. The enriched arsenate reductase gene (arrA) sequences were closest to sequences in known arsenate reducing Sulfurospirillum barnesii. This study bridges the knowledge gap between cultivation-independent and cultivation-dependent analyses. It clarifies the mechanism for arsenic mobilization and redistribution in rural drinking water wells, as a balance between bioremediation and harmful activities. The identified, potentially dissimilatory arsenate and iron-reducing microbes, constitute a risk for arsenic bioremediation. Appropriate metagenomic and dynamic analyses should help design and maintain safer, effective bioremediation.