Gram Positive Antibacterial Activity of Ethyl Acetate Extract of Penicillium sp. LBKURCC34's Growth Media Stimulated by Staphylococcus aureus

Abstract

This study was aimed to evaluate the antimicrobial activity of local isolate of peat swamp soil strain, Penicillium sp. LBKURCC34 on Gram positive pathogen, B. subtilis ATCC11774 and S. epidermidis ATCC12228. The fresh strain of Penicillium sp. LBKURCC34 and Staphylococcus aureus was added into fermentation media and incubated for 14 days. The media extracted with ethyl acetateand evaporated, then the concentrate dissolved in methanol. Antimicrobe test was performed by the disc diffusion method and the resazurin-based turbidometric method toward Gram positive bacterial pathogens (Bacillus subtilis ATCC11774 and Staphylococcus epidermidis ATCC12228). The crude extract could inhibit the growth of these pathogen microorganism. The extract with biotic stimulation has higher activity than the extract without biotic stimulation. It could give 10% higher activity against Bacillus subtilis ATCC11774 compared with positive control, amoxicillin. This fungal strain is potential as antibiotics producer.