ASSAY OF MALT β‐GLUCANASE USING AZO‐BARLEY GLUCAN: AN IMPROVED PRECIPITANT

Abstract

A procedure recently described for the assay of malt β‐glucanase, which employs a dye‐labelled and chemically‐modified barley β‐glucan substrate, has been improved by changing the precipitant solution used to terminate the reaction. The new precipitant solution contains 0·4% (w/v) zinc acetate and 4% (w/v) sodium acetate dissolved in 80% (v/v) aqueous methyl cellosolve. With this precipitant the procedure can be directly applied to the assay of cellulase activity, and with minor modification, to the assay of lichenase activity. 1987 The Institute of Brewing & Distilling