Molecular diagnosis of cutaneous leishmaniasis in an endemic area of Acre state in the Amazonian region of Brazil

Abstract

Introduction: This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre). Methods: Skin biopsy imprints or biopsy fragments were assayed via kDNAPCR/ RFLP and FRET-real-time PCR. Results: Of individuals with suspected CL, 18 were positive for Leishmania kDNA. Leishmania (Viannia) braziliensis (61.1%) and Leishmania (Viannia) guyanensis (5.5%) were identified in the positive samples. Conclusions: These results are congruent with the previous reports in Acre and Bolivia, revealing L. braziliensis as the most prevalent species. L. guyanensis identification also corroborates with the epidemiology of the disease in the Amazon Basin.